- The experimental design is at best, confusing since different experimental groups are tested in each data set. Why is annexin V treatment missing in D?
-The summary of the data is:
1, serum withdrawal and Cyto D reduce MafB protein and latex beads engulfment
2, annexin V reduce MafB protein
3, serum withdrawal reduces Iba1 protein
-The reduction in Iba1 could result both from transcriptional or translational changes, or by a reduction in the number of microglia, and should be discussed.
-There is no quantification of "cannibalistic efferocytosis" or "phagoptosis" whatsoever, and it should be discussed in a clearly speculative context. To reach some kind of conclusion on "cannibalistic efferocytosis" the authors must 1 quantify the number of microglia; and 2, quantify engulfment of apoptotic BV2 cells by other BV2 cells. To reach conclusions on "phagoptosis" the authors must obtain direct evidences that BV2 cells engulf live BV2 cells.
-This sentence is really confusing "Although cytochalasin D were able to bind fluorescent PS beads .."