Peer-reviewed by 2 reviewers with median rating of 16/20. Review process was triple-blinded.
Round 1 (14.5/20)
Round 2 (16/20)
Conceptual advance and Impact7
The manuscript describes the isolation, identification, and characterization of a mutant (bar18) from a phenotypic screen for abnormal number of muscle nuclei. The authors mapped the mutant to the unc-83 gene, perform rescue experiments, and identify a potential interacting partner unc-84, which is supposed to act in a complex with unc-83. The manuscript is technically sound, all experiments are well designed and executed.
There is only one minor typographical error in the methods section:
The plasmid genotypes described for dBT599 and dBT573 are identical (see the first line of section "Generation of transgenic rescue lines"). This is most likely a copy-paste error, please make suitable corrections. With this change, the manuscript appears suitable for publication.
1. The plasmid genotypes described for dBT599 and dBT573 are identical (see the first line of section "Generation of transgenic rescue lines"). This is most likely a copy-paste error, please make suitable corrections.
Conceptual advance and Impact3
Through genetics, this report shows that two genes (UNC-83, UNC-84) known to affect nuclear positioning in different tissues, also affect positioning in C. elegans muscle cells. While the emphasis is on nuclear positioning the defect in this case rather could arise from cell positioning, as the authors disclose.
The word LINCing is confusing and misleading. The first idea comes to mind is a long non coding RNA(lnc), as the acronym LINC is not very common. Consider title change spelling the acronym and using active voice.
Abstract looks good. If there are nuclear defects in the mammalian orthologs, that information should also be added to the abstract.
Phenotype on Figure 1a is not completely evident. Some extra labelling like arrows pointing at it, or finding a more representative image. Also it's not clear if the image is from anterior or posterior side of the animal.
Add references to
LINC complexes cross the nuclear membrane and are composed of SUN and KASH domain-containing proteins, which interact in the perinuclear space between the inner and outer nuclear membrane. KASH proteins are located at the outer nuclear membrane and may interact with actin filaments, microtubules (via dynein and kinesin), intermediate filaments (via spectrin), centrosomes and other cytoplasmic organelles. SUN proteins are located at the inner nuclear membrane and are associated with both chromatin and nuclear lamins. Functions include nuclear movement and anchoring, moving meiotic chromosomes and telomeres and sensing mechanic stimuli.
It could also be added that it is not know when in development the defect occurs.