The authors showed that in murine acute brain slices the internalization of two SRs ligands was very high in NeuN-positive cells and they concluded that cortical neurons express large amounts of functional SRs.
They claim that they detected very few non-neuronal cells internalizing those ligands, so it would be very useful to include those data.
In the figure, in fact, no other marker is shown (es. for astrocytes / for microglia) but NeuN.
- Also, could the authors provide higher magnification images for the NeuN staining?
- Could possibly provide a nuclear marker, such as DAPI?
It is important for the authors to include in the figure the colocalization of MBSA and polyG with Iba1 and GFAP, respectively.
This will be necessary to support their conclusion that "in cerebral cortex neurons may have higher number of functional SRs on the surface than other cell-types".
Importantly, which structure of the brain slice has been imaged?
In addition, the authors could also consider to perform in primary cortical neurons the same in vitro MBSA internalization assay that they have performed in Raw264.7 cells (FigA-B).