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Discipline
Biological
Keywords
Curiosity
Dopamine
Pet
Fallypride
Mouse
Observation Type
Standalone
Nature
Standard Data
Submitted
Dec 17th, 2016
Published
Mar 9th, 2017
  • Abstract

    Here, we examine the potential of positron emission tomography (PET), a non-invasive technique that detects the location of a small molecule within a subject in real-time with resolution in the micrometre range, in providing insight into the role of dopaminergic signalling in exploratory behaviours. Using a pilot of five adult mice, we recorded the behaviour of each subject during a 15-min free exploration period and then performed PET imaging with the F-labelled high-affinity dopamine D2/D3 receptor antagonist 18F-fallypride. A correlation matrix of behaviours and brain regions of interest revealed some interesting correlations. In particular, we find a decreased standardised uptake value (SUV) for 18F-fallypride in the hippocampal formation and amygdala in subjects that exhibited high levels of unassisted rearing. This finding suggests that either a higher concentration of dopamine in these areas, or lower D2/D3 receptor availability, is associated with increased exploratory behaviour. In contrast, we found that high SUVs for 18F-fallypride throughout the brain correlated most strongly with immobility and body grooming, suggesting these behaviours dominate during times of low global dopamine/dopamine receptor binding. This pilot study serves as an example of the potential for using tandem behaviour-PET to identify novel brain-behaviour interactions, but additional refinements to the methods are warranted before full-scale studies are engaged.

  • Figure
  • Introduction

    As a non-invasive technique that permits high-resolution real-time visualisation of receptor density and distribution with the help of radioactively-labelled small molecules, small animal positron emission tomography (PET) is an ideal tool to examine metabolic demand, receptor expression and neurotransmitter signalling in the intact brain. The general principle underlying PET is that the concentration of a trackable radiotracer depends on both the concentration of available binding sites (typically determined by receptor expression) and the amount of competing endogenous ligand. Although first developed for use in humans, PET is now also performed with non-human primates as well as smaller experimental subjects (usually rodents, termed “small animal PET”). As a result, PET is increasingly recruited to address fundamental questions in neuroscience. In particular, small animal PET has proved to be a powerful tool to determine phenotypic differences resulting from genetic modification and has been insightful in research examining ischaemia, tumorigenesis, glucose metabolism and drug-addiction. For example, exposure to an environment previously paired with cocaine leads to a reduction in striatal 11C-raclopride binding, suggesting either a downregulation of D2-like receptors or an increase in dopamine release in the striatum when a cue for the drug is presented.

    The use of small animal PET to directly study behaviour in healthy wildtype animals is far more rare, and no studies that we could find have examined correlations between the PET signal of specific receptor-binding radiotracers (as opposed to a general metabolic sensor) and the duration of time spent performing individual behaviours prior to PET imaging. Our study here was, therefore, an exploratory endeavour, aiming to determine the feasibility and potential of such an approach. We decided to focus on novelty-driven exploration since it is a fundamental behaviour governed by dopaminergic signalling, and the dopaminergic neurotransmitter system is ideal to examine with PET. We therefore monitored the behaviour of mice in a novel, non-stressful environment and then performed small animal PET using 18F-fallypride as our radioligand since it is a D2/D3 receptor-binding small molecule suitable for measurement of extrastriatal dopamine release. The aim was to investigate the potential for this approach to identify novel brain regions in which dopamine signalling may underlie exploration or be involved in important mental processes that take place during the post-exploration period (e.g. memory consolidation). Briefly, we find there is some promise, but refinements in the experimental protocol warrant investigation before full-scale studies are initiated.

  • Objective

    The general objective of this study was to examine if performing small animal PET immediately following a behavioural analysis provides any possibility to identify brain-behaviour interactions.

  • Results & Discussion

    During the 15-min free exploration period (Fig. 1A), all five mice spent at least 25% of the time actively engaged in a specific behaviour other than sitting (“Im” or “immobility”) or transitioning between behaviours ( “null”). Unassisted rearing, ranging from 0.3% to 21.0% (CV = 0.79), exhibited the most variation of the behaviours performed by all five animals (Fig. 1B). The behaviour with the least variation was object interaction (CV = 0.12). All subjects spent more time walking near the walls of the arena than in the centre, as expected, and overall behavioural performance was consistent with other recent investigations. In the subsequent PET scan, regional distribution of 18F-fallypride-binding potential among a set of regions of interest (ROIs) was consistent with previous studies, exhibiting strongest uptake in regions such as the striatum where D2/D3 receptors are highly expressed, and whole-brain standardised uptake value (SUV) variation (Fig. 1C,D) was within the typical range.

    We next used a correlation matrix to perform unbiased comparisons between the two datasets and observed both expected and unexpected outcomes (Fig. 1E).

    First, unassisted rearing (canonical exploratory behaviour performed by mice) demonstrated an inverse correlation with 18F-fallypride SUV throughout the brain, suggesting either a brain-wide internalisation of D2/D3 receptors or an increase in dopamine release throughout most of the brain. We favour the latter interpretation since novel environments like the one employed in this study are known to increase the firing rate of ventral tegmental area (VTA) dopamine neurons. What our study adds to this knowledge is the potential that the degree of VTA activation corresponds to the degree of exploration within a novel environment, even when the degree of absolute novelty remains constant. Of the many ROIs demonstrating a negative correlation between 18F-fallypride SUV and unassisted rearing, the hippocampus and amygdala showed the strongest Pearson’s r. Dopamine projections to these specific regions may, therefore, be the most strongly activated by sensorium-enriching exploratory behaviours.

    The association between dopaminergic signalling and the hippocampus helps confirm an already established interaction, but the strong correlation with the amygdala was more surprising. While VTA-amygdalar dopamine transmission is proposed to signal danger and increased dopamine release occurs in response to stress, more unassisted rearing in the safe novel environment employed here would not be expected to indicate more stress. Unassisted rearing is unlikely a proxy for danger in our experiment. More likely, the lack of 18F-fallypride in the amygdala reflects memory support mechanisms consistent with reduced amygdalar SUVs observed in humans during word pair association tasks.

    Second, we observed that SUVs within the superior and inferior colliculi did not correlate strongly with any of the scored behaviours, suggesting D2/D3 receptors in the colliculi do not play a major role in these behaviours under the conditions used in this study. The special lack of correlations for 18F-fallypride uptake in the colliculi also points towards a functional separation of dopamine neurons that project to the colliculi compared to other target regions. Both of these ideas are consistent with the seemingly specific role for dopamine signalling in the colliculi in behavioural responses to aversive or fearful stimuli. Our experiment examined well-handled subjects in a non-fearful environment. In this context, D2/D3 receptor expression level and/or dopamine release in the colliculi would not be expected to play any major role.

    Third, the strongest correlations between behaviour and 18F-fallypride SUV emerged when examining head grooming (grooming anywhere in the face and head region while standing upright on hind legs). In behavioural literature, head grooming is rarely scored on its own, even when grooming microstructure is being examined as a proxy for internal states like anxiety. However, grooming initiation is known to be governed by dopaminergic signalling, and mice always start grooming at the face. The strong correlations between global dopamine signalling and head grooming would make sense in this context. In addition, head grooming could contribute to exploration as mice sample scents collected on their paws from the environment.

    Finally, we assessed the feasibility of merging small animal PET and behavioural analyses using a Pearson’s r power analysis. Without considering multiple comparisons, sample sizes of 10 to 15 animals would be needed to obtain statistical significance for stronger correlations (e.g. hippocampus 18F-fallypride and unassisted rearing). When considering multiple comparisons, the sample size required becomes highly dependent on correlative strength. For weaker correlations in the 0.6 range, multiple comparisons inflate the required sample size up to 25-30 subjects. Stronger correlations (Pearson’s r >0.8) are hardly affected by multiple comparisons, indicating sample sizes in the 10-15 range would still be adequate.

  • Conclusions

    The current study describes the results of a correlation matrix derived from behavioural analysis in a novel safe environment and small animal PET imaging with the D2/D3 receptor radioligand 18F-fallypride. The data are promising with respect to the possibility for tandem behaviour-PET to reveal novel brain-behaviour interactions. Even with its limited parametric power, the current study identified known interactions between dopaminergic signalling in the hippocampus and unassisted exploratory rearing. This is not to say that the other specific correlations revealed in this pilot study should be considered definitive. Prior to engaging in full studies, it would be prudent to first examine additional experimental refinements, such as administering the radioligand before behaviour.

  • Limitations

    First, as is the case for all PET experiments, SUVs are determined by both the concentration of endogenous ligand and the surface expression of the target receptor. Histological assays, in vivo microdialysis and other complementary approaches are, therefore, needed to decipher between these sources of signal change.

    Second, this study is parametrically limited. Only five male mice of a single strain were employed. Direct conclusions drawn from high Pearson’s r values in the correlation matrix are strong candidates for false-positives. The primary value of the study is, therefore, not in drawing immediate conclusions on dopaminergic signalling underlying exploration but rather in assessing the possibility of doing so following a larger study.

    Finally, the inherent delay in performing tandem behaviour-PET makes it difficult to determine if any observed correlations with specific behaviours are driven by dopamine signalling during free exploration or result from post-exploration processes such as memory consolidation. This issue could, at least in part, be resolved by simultaneously performing behaviour and PET using head-fixed awake subjects behaving on a rotating ball or miniature PET equipment that can be carried directly on a rat’s head. However, these newer technologies are not without their own disadvantages, including complicated set-ups, large number of training hours needed before experimentation can begin and restricted subject manoeuvrability. We therefore felt that if insightful correlations could be made using standard small animal PET, this would represent an exciting opportunity in neuroscience.

  • Methods

    Experimental subjects

    Five young adult (8 weeks of age at study onset) B6/J-Rj male mice were obtained from Janvier (France). Prior to engaging in any experimental paradigms, the mice were allowed to acclimatise to the facility for a week. Mice were maintained on a 12/12-h light/dark cycle (lights on at 6:00 am; lights off at 6:00 pm) with access to standard chow and drinking water ad libitum throughout the study. Internally ventilated cages containing tissue for nest building, pinewood bedding and a pressed paper house were used. Mice were handled for 7 days, 10 min/day prior to the novel environment exploration and PET imaging.

    Novel environment exploration

    Protocol and conditions were as reported previously. Briefly, a topless, bottomless and colourless Plexiglas frame (40 cm3) was placed directly over a separate Plexiglas sheet on which three identical objects stood. The objects were metal chrome spheres of 2 cm diameter mounted on a flat chrome pedestal. Total object height was 3 cm. The behavioural arena was illuminated by dim overhead white light (≈100 lx inside the Plexiglas box). Object exploration (each object separately, combined for analysis), assisted rearing, unassisted rearing, head grooming, body grooming, wall walking (within 5 cm from wall), centre walking (beyond 5 cm from walls), risk assessment and immobility were recorded manually with Noldus Observer XT software (the Netherlands). Transitions between behaviours were also recorded as immobility. Extra-maze visual cues were placed within the behavioural suite (adjacent to both the animal housing room and PET imaging room). All behaviours except “risk assessment” passed a Kolmogorov-Smirnov Gaussian distribution test.

    PET imaging

    18F-fallypride was synthesised from its precursor tosyl-fallypride (ABX GmbH, Radeberg, Germany) as previously reported. In vivo 18F-fallypride-PET experiments were performed with a dedicated small animal PET/CT tomograph (Super Argus, Sedecal, Madrid, Spain) according to procedures previously described. Briefly, mice were anaesthetised with isoflurane in an air-oxygen mixture and positioned on the scanner bed. Respiratory frequency and body temperature were monitored and controlled with a monitoring system (model 1025T, SA Instruments, Stony Brook, NY). 18F-fallypride was injected via a lateral tail vein with 8-15 MBq (9-25 nmol/kg). Two minutes after radiotracer injection, PET acquisition was started in dynamic list mode for 60 min followed by CT scan. After the PET/CT scan, the animals were sacrificed and brains were dissected for future in vitro analyses. PET raw data were reconstructed in user-defined time frames with a voxel size of 0.3875 × 0.3875 × 0.775 mm by 2-dimensional ordered subsets expectation maximisation. Random and single but no attenuation correction was applied. Image files were analysed with PMOD 3.6 software (PMOD Technologies Ltd., Zurich, Switzerland). SUVs were calculated from the image-derived radioactivity in Becquerel (Bq) per centimetre tissue divided by the radioactivity dose in Bq per gram body weight, assuming a tissue density of 1 g/cm. The binding potential (BPnd) was calculated by the Logan reference tissue analysis as previously reported and PET images of the BPnd were generated with the PXMod module of PMOD.

    Statistical analysis

    The correlation matrix was created using behaviopy, a script suite that can calculate Pearson’s r for combinations of brain region intensities and behaviour times. Internally the script suite uses NumPy and SciPy for computations, Pandas for data management, and Matplotlib for plotting. For behaviour, the entire 15-min exploration period was used for analysis. For 18F-fallypride-PET, the 40-60-min time point post-18F-fallypride injection was used, since it was found to be least affected by variations in injection efficiency.

  • Funding statement

    This work was supported by generous grants from the Swiss National Science Foundation (PZ00P3_148114/1), the University of Zurich (FK-13-048), the Brain and Behavior Research Foundation (19213) and the Neuroscience Center Zurich awarded to BJS.

  • Acknowledgements

    AMH, SMA and BJS designed experiments. AMH, SB and BJS performed experiments. AMH, H-II and BJS analysed data. All authors discussed the results, contributed to the manuscript and declare no conflicts of interests.

  • Ethics statement

    All experiments and manipulations conformed to the guidelines set by the Animal Care Commission of Switzerland and were covered under the authority of animal permit ZH263/2014. All possible measures were taken to ensure minimal pain and discomfort.

  • References
  • 1
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    Matters18/30

    Using tandem behaviour-PET to examine dopaminergic signalling underlying exploration

    Abstractlink

    Here, we examine the potential of positron emission tomography (PET), a non-invasive technique that detects the location of a small molecule within a subject in real-time with resolution in the micrometre range, in providing insight into the role of dopaminergic signalling in exploratory behaviours. Using a pilot of five adult mice, we recorded the behaviour of each subject during a 15-min free exploration period and then performed PET imaging with the F-labelled high-affinity dopamine D2/D3 receptor antagonist 18F-fallypride. A correlation matrix of behaviours and brain regions of interest revealed some interesting correlations. In particular, we find a decreased standardised uptake value (SUV) for 18F-fallypride in the hippocampal formation and amygdala in subjects that exhibited high levels of unassisted rearing. This finding suggests that either a higher concentration of dopamine in these areas, or lower D2/D3 receptor availability, is associated with increased exploratory behaviour. In contrast, we found that high SUVs for 18F-fallypride throughout the brain correlated most strongly with immobility and body grooming, suggesting these behaviours dominate during times of low global dopamine/dopamine receptor binding. This pilot study serves as an example of the potential for using tandem behaviour-PET to identify novel brain-behaviour interactions, but additional refinements to the methods are warranted before full-scale studies are engaged.

    Figurelink

    Correlations of mouse behaviours in a novel, non-stressful environment with the regional distribution of 18F-fallypride in the brain.

    (A) Experimental study design. (B) Individual behaviours performed by the five mice in the novel environment. (C) Mean 18F-fallypride standardised uptake values (SUVs) in various regions of interest (ROIs) for the 40-60-min post-injection period. (D) Maximum intensity projections of 18F-fallypride binding potential immediately after a 15-min exposure to the novel, non-stressful environment. (E) Correlation matrix of duration spent on specific behaviours and 18F-fallypride SUV.

    Abbreviations: AR: Assisted rearing; UR: Unassisted rearing; WW: Wall walk; CW: Centre walk; Obj: Object interaction; BG: Body grooming; HG: Head grooming; RA: Risk assessment; Im/null: Immobility/null; Amy: Amgydala; BFS: Basal forebrain/septum; BS: Brain stem; CPu: Caudate putamen (striatum); Cg: Central gyrus; Cb: Cerebellum; IC: Colliculus, inferior; SC: Colliculus, superior; Ctx: cortex; HPF: Hippocampal formation; HT: Hypothalamus; MB: Midbrain; Ob: Olfactory bulb; Tons: Thalamus.

    Individual data points and mean ± SEM are shown.

    Introductionlink

    As a non-invasive technique that permits high-resolution real-time visualisation of receptor density and distribution with the help of radioactively-labelled small molecules, small animal positron emission tomography (PET) is an ideal tool to examine metabolic demand, receptor expression and neurotransmitter signalling in the intact brain[1]. The general principle underlying PET is that the concentration of a trackable radiotracer depends on both the concentration of available binding sites (typically determined by receptor expression) and the amount of competing endogenous ligand. Although first developed for use in humans, PET is now also performed with non-human primates as well as smaller experimental subjects (usually rodents, termed “small animal PET”). As a result, PET is increasingly recruited to address fundamental questions in neuroscience. In particular, small animal PET has proved to be a powerful tool to determine phenotypic differences resulting from genetic modification[2][3] and has been insightful in research examining ischaemia, tumorigenesis, glucose metabolism and drug-addiction. For example, exposure to an environment previously paired with cocaine leads to a reduction in striatal 11C-raclopride binding, suggesting either a downregulation of D2-like receptors or an increase in dopamine release in the striatum when a cue for the drug is presented[4].

    The use of small animal PET to directly study behaviour in healthy wildtype animals is far more rare, and no studies that we could find have examined correlations between the PET signal of specific receptor-binding radiotracers (as opposed to a general metabolic sensor) and the duration of time spent performing individual behaviours prior to PET imaging. Our study here was, therefore, an exploratory endeavour, aiming to determine the feasibility and potential of such an approach. We decided to focus on novelty-driven exploration since it is a fundamental behaviour governed by dopaminergic signalling[5][6], and the dopaminergic neurotransmitter system is ideal to examine with PET. We therefore monitored the behaviour of mice in a novel, non-stressful environment and then performed small animal PET using 18F-fallypride as our radioligand since it is a D2/D3 receptor-binding small molecule suitable for measurement of extrastriatal dopamine release[7]. The aim was to investigate the potential for this approach to identify novel brain regions in which dopamine signalling may underlie exploration or be involved in important mental processes that take place during the post-exploration period (e.g. memory consolidation). Briefly, we find there is some promise, but refinements in the experimental protocol warrant investigation before full-scale studies are initiated.

    Objectivelink

    The general objective of this study was to examine if performing small animal PET immediately following a behavioural analysis provides any possibility to identify brain-behaviour interactions.

    Results & Discussionlink

    During the 15-min free exploration period (Fig. 1A), all five mice spent at least 25% of the time actively engaged in a specific behaviour other than sitting (“Im” or “immobility”) or transitioning between behaviours ( “null”). Unassisted rearing, ranging from 0.3% to 21.0% (CV = 0.79), exhibited the most variation of the behaviours performed by all five animals (Fig. 1B). The behaviour with the least variation was object interaction (CV = 0.12). All subjects spent more time walking near the walls of the arena than in the centre, as expected, and overall behavioural performance was consistent with other recent investigations[6][8]. In the subsequent PET scan, regional distribution of 18F-fallypride-binding potential among a set of regions of interest (ROIs) was consistent with previous studies, exhibiting strongest uptake in regions such as the striatum where D2/D3 receptors are highly expressed, and whole-brain standardised uptake value (SUV) variation (Fig. 1C,D) was within the typical range[9].

    We next used a correlation matrix to perform unbiased comparisons between the two datasets and observed both expected and unexpected outcomes (Fig. 1E).

    First, unassisted rearing (canonical exploratory behaviour performed by mice[6][10]) demonstrated an inverse correlation with 18F-fallypride SUV throughout the brain, suggesting either a brain-wide internalisation of D2/D3 receptors or an increase in dopamine release throughout most of the brain. We favour the latter interpretation since novel environments like the one employed in this study are known to increase the firing rate of ventral tegmental area (VTA) dopamine neurons[11]. What our study adds to this knowledge is the potential that the degree of VTA activation corresponds to the degree of exploration within a novel environment, even when the degree of absolute novelty remains constant. Of the many ROIs demonstrating a negative correlation between 18F-fallypride SUV and unassisted rearing, the hippocampus and amygdala showed the strongest Pearson’s r. Dopamine projections to these specific regions may, therefore, be the most strongly activated by sensorium-enriching exploratory behaviours.

    The association between dopaminergic signalling and the hippocampus helps confirm an already established interaction[5][6], but the strong correlation with the amygdala was more surprising. While VTA-amygdalar dopamine transmission is proposed to signal danger[12] and increased dopamine release occurs in response to stress[13], more unassisted rearing in the safe novel environment employed here would not be expected to indicate more stress. Unassisted rearing is unlikely a proxy for danger in our experiment. More likely, the lack of 18F-fallypride in the amygdala reflects memory support mechanisms consistent with reduced amygdalar SUVs observed in humans during word pair association tasks[14].

    Second, we observed that SUVs within the superior and inferior colliculi did not correlate strongly with any of the scored behaviours, suggesting D2/D3 receptors in the colliculi do not play a major role in these behaviours under the conditions used in this study. The special lack of correlations for 18F-fallypride uptake in the colliculi also points towards a functional separation of dopamine neurons that project to the colliculi compared to other target regions. Both of these ideas are consistent with the seemingly specific role for dopamine signalling in the colliculi in behavioural responses to aversive or fearful stimuli[15][16]. Our experiment examined well-handled subjects in a non-fearful environment. In this context, D2/D3 receptor expression level and/or dopamine release in the colliculi would not be expected to play any major role.

    Third, the strongest correlations between behaviour and 18F-fallypride SUV emerged when examining head grooming (grooming anywhere in the face and head region while standing upright on hind legs). In behavioural literature, head grooming is rarely scored on its own, even when grooming microstructure is being examined as a proxy for internal states like anxiety[17][18]. However, grooming initiation is known to be governed by dopaminergic signalling[19], and mice always start grooming at the face. The strong correlations between global dopamine signalling and head grooming would make sense in this context. In addition, head grooming could contribute to exploration as mice sample scents collected on their paws from the environment.

    Finally, we assessed the feasibility of merging small animal PET and behavioural analyses using a Pearson’s r power analysis[20]. Without considering multiple comparisons, sample sizes of 10 to 15 animals would be needed to obtain statistical significance for stronger correlations (e.g. hippocampus 18F-fallypride and unassisted rearing). When considering multiple comparisons, the sample size required becomes highly dependent on correlative strength. For weaker correlations in the 0.6 range, multiple comparisons inflate the required sample size up to 25-30 subjects. Stronger correlations (Pearson’s r >0.8) are hardly affected by multiple comparisons, indicating sample sizes in the 10-15 range would still be adequate.

    Conclusionslink

    The current study describes the results of a correlation matrix derived from behavioural analysis in a novel safe environment and small animal PET imaging with the D2/D3 receptor radioligand 18F-fallypride. The data are promising with respect to the possibility for tandem behaviour-PET to reveal novel brain-behaviour interactions. Even with its limited parametric power, the current study identified known interactions between dopaminergic signalling in the hippocampus and unassisted exploratory rearing. This is not to say that the other specific correlations revealed in this pilot study should be considered definitive. Prior to engaging in full studies, it would be prudent to first examine additional experimental refinements, such as administering the radioligand before behaviour.

    Limitationslink

    First, as is the case for all PET experiments, SUVs are determined by both the concentration of endogenous ligand and the surface expression of the target receptor. Histological assays, in vivo microdialysis and other complementary approaches are, therefore, needed to decipher between these sources of signal change.

    Second, this study is parametrically limited. Only five male mice of a single strain were employed. Direct conclusions drawn from high Pearson’s r values in the correlation matrix are strong candidates for false-positives. The primary value of the study is, therefore, not in drawing immediate conclusions on dopaminergic signalling underlying exploration but rather in assessing the possibility of doing so following a larger study.

    Finally, the inherent delay in performing tandem behaviour-PET makes it difficult to determine if any observed correlations with specific behaviours are driven by dopamine signalling during free exploration or result from post-exploration processes such as memory consolidation. This issue could, at least in part, be resolved by simultaneously performing behaviour and PET using head-fixed awake subjects behaving on a rotating ball[21] or miniature PET equipment that can be carried directly on a rat’s head[22]. However, these newer technologies are not without their own disadvantages, including complicated set-ups, large number of training hours needed before experimentation can begin and restricted subject manoeuvrability. We therefore felt that if insightful correlations could be made using standard small animal PET, this would represent an exciting opportunity in neuroscience.

    Methodslink

    Experimental subjects

    Five young adult (8 weeks of age at study onset) B6/J-Rj male mice were obtained from Janvier (France). Prior to engaging in any experimental paradigms, the mice were allowed to acclimatise to the facility for a week. Mice were maintained on a 12/12-h light/dark cycle (lights on at 6:00 am; lights off at 6:00 pm) with access to standard chow and drinking water ad libitum throughout the study. Internally ventilated cages containing tissue for nest building, pinewood bedding and a pressed paper house were used. Mice were handled for 7 days, 10 min/day prior to the novel environment exploration and PET imaging.

    Novel environment exploration

    Protocol and conditions were as reported previously[8]. Briefly, a topless, bottomless and colourless Plexiglas frame (40 cm3) was placed directly over a separate Plexiglas sheet on which three identical objects stood. The objects were metal chrome spheres of 2 cm diameter mounted on a flat chrome pedestal. Total object height was 3 cm. The behavioural arena was illuminated by dim overhead white light (≈100 lx inside the Plexiglas box). Object exploration (each object separately, combined for analysis), assisted rearing, unassisted rearing, head grooming, body grooming, wall walking (within 5 cm from wall), centre walking (beyond 5 cm from walls), risk assessment and immobility were recorded manually with Noldus Observer XT software (the Netherlands). Transitions between behaviours were also recorded as immobility. Extra-maze visual cues were placed within the behavioural suite (adjacent to both the animal housing room and PET imaging room). All behaviours except “risk assessment” passed a Kolmogorov-Smirnov Gaussian distribution test.

    PET imaging

    18F-fallypride was synthesised from its precursor tosyl-fallypride (ABX GmbH, Radeberg, Germany) as previously reported[23]. In vivo 18F-fallypride-PET experiments were performed with a dedicated small animal PET/CT tomograph (Super Argus, Sedecal, Madrid, Spain) according to procedures previously described[24]. Briefly, mice were anaesthetised with isoflurane in an air-oxygen mixture and positioned on the scanner bed. Respiratory frequency and body temperature were monitored and controlled with a monitoring system (model 1025T, SA Instruments, Stony Brook, NY). 18F-fallypride was injected via a lateral tail vein with 8-15 MBq (9-25 nmol/kg). Two minutes after radiotracer injection, PET acquisition was started in dynamic list mode for 60 min followed by CT scan. After the PET/CT scan, the animals were sacrificed and brains were dissected for future in vitro analyses. PET raw data were reconstructed in user-defined time frames with a voxel size of 0.3875 × 0.3875 × 0.775 mm by 2-dimensional ordered subsets expectation maximisation. Random and single but no attenuation correction was applied. Image files were analysed with PMOD 3.6 software (PMOD Technologies Ltd., Zurich, Switzerland). SUVs were calculated from the image-derived radioactivity in Becquerel (Bq) per centimetre tissue divided by the radioactivity dose in Bq per gram body weight, assuming a tissue density of 1 g/cm. The binding potential (BPnd) was calculated by the Logan reference tissue analysis as previously reported[25] and PET images of the BPnd were generated with the PXMod module of PMOD.

    Statistical analysis

    The correlation matrix was created using behaviopy, a script suite that can calculate Pearson’s r for combinations of brain region intensities and behaviour times[26]. Internally the script suite uses NumPy and SciPy for computations, Pandas for data management, and Matplotlib for plotting. For behaviour, the entire 15-min exploration period was used for analysis. For 18F-fallypride-PET, the 40-60-min time point post-18F-fallypride injection was used, since it was found to be least affected by variations in injection efficiency.

    Funding Statementlink

    This work was supported by generous grants from the Swiss National Science Foundation (PZ00P3_148114/1), the University of Zurich (FK-13-048), the Brain and Behavior Research Foundation (19213) and the Neuroscience Center Zurich awarded to BJS.

    Acknowledgementslink

    AMH, SMA and BJS designed experiments. AMH, SB and BJS performed experiments. AMH, H-II and BJS analysed data. All authors discussed the results, contributed to the manuscript and declare no conflicts of interests.

    Conflict of interestlink

    The authors declare no conflicts of interest.

    Ethics Statementlink

    All experiments and manipulations conformed to the guidelines set by the Animal Care Commission of Switzerland and were covered under the authority of animal permit ZH263/2014. All possible measures were taken to ensure minimal pain and discomfort.

    No fraudulence is committed in performing these experiments or during processing of the data. We understand that in the case of fraudulence, the study can be retracted by Matters.

    Referenceslink
    1. Xi Wang, Tian Mei, Zhang Hong
      Molecular imaging in neuroscience research with small-animal PET in rodents
      Neuroscience Research, 70/2011, pages 133-143 DOI: 10.1016/j.neures.2010.12.017chrome_reader_mode
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