The immobility periods of the mice were measured and mean values were compared between the vehicle control group and each of the groups who were administered with the test drug. Compared with the vehicle control group, all treated groups showed a significant decrease in the immobility time. The experiment yielded the following observations: positive control group (P = 7.42E-05), reagent S-1 group (P = 0.0060), reagent H-1 group (P = 0.0004), and reagent K-1 group (P = 0.005) (Fig. 3 and Table. 1)
This experiment shows that imipramine, the positive control substance, yielded a significant reduction in the immobility time. Therefore, this experiment was considered to have been established as an anti-depression test model. Moreover, S-1, H-1, and K-1 were observed to significantly decrease the immobility time.
The above results suggest that reagents S-1, H-1, and K-1 demonstrated an anti-depressant effect
Reagent name/Weight of the diluting agent used /Name of constituents in pure form/Weight in the pure form
Reagent S-1/300 mg/kg /Sumatriptan/39 mg/kg
Reagent H-1/250 mg/kg/Diphenhydramine /9 mg/kg
Reagent K-1/150 mg/kg/Tolperisone hydrochloride/56 mg/kg
Principles of Depression
Depression is considered to be a disorder of the mind/heart as well as the brain. However, this disorder is more complex and cannot be completely understood easily. Depression is “a disorder that affects the entire neck and head region above the Adam’s apple”. In addition, depression is not simply the presence of one symptom, i.e., “decreased serotonin or lack of serotonin.” If depression had just one symptom, a cure would have been found after conducting several experimental studies. However, a cure cannot be found as easily if there is a combination of 2–3 symptoms. In other words, depression is “an anomaly of 3 major regions” and “a combination of 2–3 symptoms associated with them.” The concept of depression needs to be clarified by addressing these principles.
The three kinds of drugs for the treatment of depression
As per the current standards, only one type of drug is recommended to treat depression; however, this is inadequate. If one type of drug does not yield any effect, simultaneous administration of several drugs at random also tends to be ineffective. In this report, the authors propose a set of three types of drugs to treat depression—this is extremely important. It is apparent that the dose and administration period of the drugs necessary is different for the treatment of each symptom, and consequently, the improvement they bring about will be different. It is better to treat one symptom at a time in a systematic manner.
The following is a list of constituents of the three types of drugs:
Constituents of reagent S-1/Sumatriptan, 5-hydroxytryptamine, almotriptan, eletriptan, naratriptan, and lmitriptan
Constituents of reagent H-1/Diphenhydramine, chlorpheniramine, clemastine, alimemazine,and hydroxyethylpromethazine,
Constituents of reagent K-1/Tolperisone and tizanidine