Peer-reviewed by 2 reviewers with median rating of 16.5/20. Review process was triple-blinded.
Round 1 (16.5/20)
Conceptual advance and Impact9
Exceptionally well written paper. Interesting observation. I think it's a perfect fit for Science Matters.
I would recommend not using so many abbreviations in the abstract and throughout the paper. They are not conventional and it is quite easy to lose track of the narrative because of the need to keep everything in mind.
Conceptual advance and Impact8
This is a good observation, if not slightly limited in information around the technique. I think it's quite important for them to explain how the dye stains the PVs and not the LVs, as this is a fundamental concept that underlies this finding.
I would explain why you think the * marks a point of rupture and not a point of entry.
"The dyes Trypan Blue and Alcian Blue have been found to help in identifying the PVS in vivo due to the dyes’ strong ability to stain the PVS."
- Please provide some explanation how this happens mechanistically and why it stains the PVS specifically. This is important for readers to understand the basis of this finding.
Results & Discussion
Given that these vessels are very difficult to identify, some suggestion about how others may improve their ability to locate them might be useful. Particularly given your article makes a push to ask other groups to take up the fight of finding these PVs!
You mention immunohistochemistry, but what exactly would you target? Do you know what markers may or may not be on the PVs? You should allude to some potential markers if the suggestion is to use IHC for staining.
Was this mouse used specifically for this purpose? In other words, was this mouse prepared *specifically* for you to try and identify a PV? This has ramifications for others who plan to follow up this work.