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Discipline
Biological, Medical
Keywords
Serotonin
Aging Mouse
Aging
Cerebellum
Observation Type
Standalone
Nature
Standard Data
Submitted
Jan 23rd, 2017
Published
Mar 9th, 2017
  • Abstract
    Mobility and locomotor impairments have high prevalence, morbidity, and significant mortality in older adult populations. Cerebellar functional changes have been implicated in the pathogenesis of these age-related mobility and gait deficits unrelated to stroke, Parkinson’s disease, or degenerative joint disease. We thus examined total cerebellar glutamate, glutamine, GABA, glycine, dopamine, norepinephrine, tryptophan, serotonin, alanine, threonine, and asparagine content from male 2-3-month (young, 6) and 21-24-month-old (aged, 6) C57BL/6 mice. Neurotransmitter and amino acid concentrations were determined by high-performance liquid chromatography followed with mass spectroscopy. We found a significant increase in cerebellar serotonin in aged versus young mice, but otherwise no significant phenotypic differences in measured neurotransmitter concentrations. Applying current thought about cerebellar aging and cerebellar serotonergic systems, we consider how this age-related increase in cerebellar serotonin may contribute to gait ataxia.
  • Figure
  • Introduction
    It is essential to understand how aging affects mobility, and find interventions to prevent or delay mobility, impairments. Mobility impairments often accompany advanced age. They are major causes of morbidity in the “oldest old,” the most rapidly expanding demographic group in the United States. Gait speed is sensitive biomarker of overall functional status, as well as a powerful predictor of overall mortalityCerebellar dysfunction may be a potential source of age-related mobility impairments. In humans, overall cerebellar volume decreases with age as shown by voxel-based morphometry and is associated with age-related gait impairments including slow gait speed. Aging is accompanied by a marked change in cerebellar gene expression patterns in both rodent models and humans. No age-related decrease in cerebellar granule cell number or density has been noted in rodent models; small decreases have been described in humans. Aging is also associated with a strain-specific increase in excitatory synaptic puncta in the internal granule cell layer; however, these synapses show deficits in excitatory amino acid neurotransmission. Loss of visualized synaptic boutons on Purkinje cell dendritic networks also accompanies age-related Purkinje cell involution and loss. Aging also evokes deficits in Purkinje cell function, including marked slowing of firing rates and blunted responses to adrenergic stimulation. These changes have been correlated with impairment in mobility-related behaviors, including walking on a runway and dynamic balance.
  • Objective
    To further explore potential synaptic deficits underlying age-related gait impairments, we assessed whole-cerebellum neurotransmitter and excitatory amino acid content in cohorts of young and aged C57BL/6 mice using HPLC mass spectroscopy (HPLC-MS). We report a novel finding of increased whole cerebellum serotonin in aged, compared to young, C57BL/6 mice.
  • Results & Discussion
    Results
    All samples were suitable for HPLC-MS determination of cerebellar neurotransmitter and amino acid levels: glutamate, GABA, glycine, glutamine, dopamine, norepinephrine, tryptophan, serotonin, alanine, threonine, and asparagine. Our results suggest a 1.4-fold statistically significant increase in whole-cerebellar serotonin in aged C57BL/6 mice compared to young conspecifics (Figure a). We otherwise found no other differences in whole cerebellum neurotransmitter or amino acid concentrations between the young and old mouse cohorts.
    Discussion
    Serotonin is an ancient regulator of cellular function, and has a coordinating role in the performance of movement behaviors across a wide variety of organismsAging has a clear impact on cerebellar serotonergic function. The activity of tryptophan hydroxylase, the enzyme catalyzing the rate-limiting conversion of tryptophan to serotonin, is significantly decreased in the midbrain and pons of aged rats. Concordantly, age-associated decreases in whole cerebellar serotonin have been reported in rats (assayed by a fluorometric reaction between serotonin and o-phthaldialdehyde) and senescence-accelerated mice (assessed by 3H-tryptophan injection). However, no age-associated differences in cerebellar serotonin content were noted among cohorts of 6-24-month-old BALB mice (assayed by HPLC), and our study is the first to report an age-associated increase in cerebellar serotonin content in a mouse model. Multiple studies have also demonstrated a marked loss of serotonin reuptake transporter function in the cerebellum of older adults.
    Serotonin has its most profound effect on the Lugaro inhibitory interneurons of the internal granule cell layer. These cells are numerous, have strong inhibitory projections onto Golgi cell inhibitory interneurons, and are typically silent. Lugaro cells respond to serotonin (likely through a 5-HT
    2 subfamily receptor) with a marked increase in spiking activity leading to increased inhibitory postsynaptic potentials onto Golgi cell membranes. Lugaro cell activity also directly inhibits Purkinje neuron firing. A single Lugaro cell makes inhibitory synaptic contact with about 150 nearby Golgi cells; each Golgi cell makes inhibitory synaptic contact with about 3,000-5,000 granule cells. Lugaro cell activity thus has the potential to reduce both the tonic and phasic components of Golgi cell inhibition onto large populations of granule cells. Serotonergic neurotransmission also contributes more subtle effects on cerebellar input signal processing. Serotonin attenuates parallel fiber input onto Purkinje cells (both by increasing activity of inhibitory basket and stellate interneurons and by presynaptic decreases of granule cell glutamate). Serotonin also modulates cerebellar deep nuclei function in a complex, incompletely understood manner.
  • Limitations
    Our results suggest an age-related 1.4-fold increase in whole cerebellar serotonin content. The methods we employed do not allow us to further determine if this difference reflects increased intravesicular serotonin within cerebellar serotonergic projections, and/or increased extracellular serotonin. Similarly, we cannot determine if this increase occurs over the cerebellum as a whole, or preferentially affects either the cerebellar input regions (internal granule cell, molecular, and Purkinje layers) or the cerebellar output nuclei; nor can we determine whether this finding localizes to specific folia.
  • Conjectures

    The predicted outcome of increasing cerebellar serotonergic activity may thus be to increase granule cell input sensitivity to mossy fiber input, decrease Purkinje cell sensitivity to parallel fiber input, and increase inhibition of Purkinje cell firing. These effects would combine to expand mossy fiber input participating in center-surround inhibition. In other words, increased serotonergic activity may allow a larger set of afferent sensory inputs concurrent access to the Purkinje cell network while preserving overall Purkinje cell firing properties. Of note, in normal human volunteers, increased extracellular serotonin (through a single dose of a selective serotonin uptake inhibitor) evoked a significant increase in resting-state fMRI centrality measures across the cerebellum, consistent with the hypothesis that increased serotonergic activity enhances cerebellar functional connectivity.

    It is thus interesting to note in this context that inhibition of serotonergic tone (by treatment with the partial 5-HT1AR agonist buspirone) improved cerebellar tremor. Further, prominent cerebellar ataxia has been observed in aged (21-24 monthold) compared to young (2-3 months old) C57BL/6 mice obtained from the same colony as the mice in this study. Interestingly, this appeared to be strain-specific with no ataxia present in BALB mice of identical ages. Locomotor ataxia thus accompanies the increased cerebellar serotonin content observed in C57BL/6 mice, but not in BALB mice with no age-related changes in cerebellar serotonin content. Gait ataxia may potentially be a behavior evoked by serotonergic increases in cerebellar functional connectivity. Further studies to determine if age-associated increases in cerebellar serotonin content are reflected in extracellular serotonin concentrations, and to determine the specific serotonin receptor subtypes associated with Lugaro cell activation, will better define the mechanisms through which serotonergic activity may influence cerebellar tremor, as well as identify potential therapeutic targets.
  • Methods

    Animals

    All studies were performed in accordance with the NIH Guide for the Care and Use of Laboratory Animals and were approved by our Institutional Animal Care and Use Committee. Six young male C57BL/6J mice were obtained from Jackson Laboratories at 8 weeks of age. Six old (20 months) male C57BL/6 mice were obtained from the National Institute of Aging rodent facility. Animals were housed in our vivarium for 1 month in microisolator cages (Lab Products Inc., Seaford, DE). Mice experienced a 12:12 lighting cycle (lights on 0600 CST) and were provided with chow (Envigo Teklad #7012) and water ad libitum.

    Tissue processing

    Mice were euthanized with acute CO2 asphyxiation, followed by rapid decapitation. Brain was isolated from the skull and placed into ice-cold PBS (Hyclone SH30256.01). Under a dissecting microscope, cerebellum was separated from brain and flash-frozen in isopentane for 5-10 s. Frozen cerebellums were stored at -80°C in canonical tubes until shipping, at which time the tissues/tubes were packed in dry ice and shipped overnight to Brains Online for further analysis.

    Sample preparation

    Mouse cerebella were homogenized using a Misonix Sonicator 3000 ultrasonic cell disruptor (Qsonica, LLC, Newton, USA). Per mg cerebellum 4 μL of 0.5 M perchloric acid was added as tissue destruction matrix. After homogenization, the samples were spun down and supernatant was processed further for the quantification of glutamate, GABA, glycine, glutamine, tryptophan, dopamine, norepinephrine, serotonin, alanine, asparagine, and threonine.

    Analysis

    Supernatant was further processed and 20 μL of the processed sample was mixed with 4 μL stable labeled internal standards mix of the analytes of interest. The mixture was derivatized with the proprietary SymDAQ reagent (BrainLink, Groningen, NL) in the autosampler part of an integrated LC system (prominence series; Shimadzu, Japan) by addition of 30 μL SymDAQ reagent solution to the sample vial. After the reaction, 45 μL of the mixture was injected onto the HPLC-MS system. Chromatographic separation was performed on a reverse-phase column (100×3.0 mm, 2.5 μm particle size; Synergi MAX-RP, Phenomenex, Torrance, USA) at 30°C. Sample analytes were separated using a 100 to 0% gradient of mobile phase A (ultrapure water/acetonitrile (98/2), 0.1% formic acid) to mobile phase B (ultrapure water/acetonitrile (30/70), 0.1% formic acid) at a flow rate of 300 μl/min. A post-column make-up flow of 150 μL/min consisting of 90% acetonitrile and 10% water, was added to the flow of the HPLC, prior to entering the MS for analyte detection. MS analysis was performed using a system consisting of an API 4000 triple quadropole detector and a Turbo V Ion Spray interface (AB Sciex, Redwood City, USA). The acquisitions were performed in positive ionization mode, with ionization spray voltage set at 3 kV and a probe temperature of 200°C. The instrument was operated in multiple reaction monitoring mode. The collision gas (nitrogen) pressure was held at 2 psig. Data was calibrated and quantified using the Analyst™ version 1.4.2 data system (AB Sciex, Redwood City, USA).

    Statistical analysis

    Neurotransmitter/amino acid concentrations were compared over the two cohorts using one-way analysis of variance, with Bonferroni corrections applied to adjust the critical p value for eight simultaneous comparisons (we did not include alanine or threonine in our calculations since these amino acids are not neurotransmitters or neurotransmitter precursors in the cerebellum). Statistics were calculated using MATLAB (anova1; Mathworks, Natick, MA).

  • Funding statement
    This study was supported by NIH/NIA R01-AG031158 to SJB.
  • Acknowledgements
    The authors thank Ms. Tammy Chaudoin for her expert technical support.
  • Ethics statement
    Not applicable.
  • References
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    Matters21.5/30

    Increased whole cerebellar serotonin in aged C57BL/6 mice

    Abstractlink
    Mobility and locomotor impairments have high prevalence, morbidity, and significant mortality in older adult populations. Cerebellar functional changes have been implicated in the pathogenesis of these age-related mobility and gait deficits unrelated to stroke, Parkinson’s disease, or degenerative joint disease. We thus examined total cerebellar glutamate, glutamine, GABA, glycine, dopamine, norepinephrine, tryptophan, serotonin, alanine, threonine, and asparagine content from male 2-3-month (young, 6) and 21-24-month-old (aged, 6) C57BL/6 mice. Neurotransmitter and amino acid concentrations were determined by high-performance liquid chromatography followed with mass spectroscopy. We found a significant increase in cerebellar serotonin in aged versus young mice, but otherwise no significant phenotypic differences in measured neurotransmitter concentrations. Applying current thought about cerebellar aging and cerebellar serotonergic systems, we consider how this age-related increase in cerebellar serotonin may contribute to gait ataxia.
    Figurelink
    Figure a. Age-evoked increase in whole cerebellar serotonin in C57BL/6 mice.
    Boxplots provided for whole cerebellum neurotransmitter concentrations; data points superimposed upon boxplots. Glutamate, GABA, glutamine, tryptophan, and norepinephrine values correspond to the y-axis valued 0-10; dopamine values correspond to the y-axis labeled 0-100; glycine and serotonin values correspond to the y-axis labeled 0-1,000. Neurotransmitter concentration units provided on the x-axis. Blue dots depict measures from the young (2-3 months) cohort; green dots depict measures from the old (21- 24 months) cohort.


    For all comparisons, age df = 1, error df = 10, total df = 11. Respective F and p values are as follows: Glu F1,10 = 1.13, = 0.3131; GABA F1,10 = 1.64, = 0.229; Gln F1,10 = 0.38, = 0.5495; Gly F1,10 = 6.85, = 0.0257; Ala F1,10 = 0.44, = 0.52; Thr F1,10 = 3.8, = 0.0799; Trp F1,10 = 3.13, = 0.1073; Asn F1,10 = 2.9. = 0.1192; DA F1,10 = 0.08, = 0.7863; NE F1,10 = 0.03, = 0.8665; 5-HT (*) F1,10 = 13.53, = 0.0043. Critical = 0.0063 to achieve α = 0.05 for Bonferroni correction over eight comparisons.


    The MATLAB program used to generate the figures as well as to run ANOVA statistics can be found in the supplementary data.

    Introductionlink
    It is essential to understand how aging affects mobility, and find interventions to prevent or delay mobility, impairments. Mobility impairments often accompany advanced age[1]. They are major causes of morbidity in the “oldest old,” the most rapidly expanding demographic group in the United States[2][3][4]. Gait speed is sensitive biomarker of overall functional status[5], as well as a powerful predictor of overall mortality[6][7]Cerebellar dysfunction may be a potential source of age-related mobility impairments. In humans, overall cerebellar volume decreases with age as shown by voxel-based morphometry[8] and is associated with age-related gait impairments including slow gait speed[9]. Aging is accompanied by a marked change in cerebellar gene expression patterns in both rodent models and humans[10][11]. No age-related decrease in cerebellar granule cell number or density has been noted in rodent models[12][13]; small decreases have been described in humans[14]. Aging is also associated with a strain-specific increase in excitatory synaptic puncta in the internal granule cell layer; however, these synapses show deficits in excitatory amino acid neurotransmission[10]. Loss of visualized synaptic boutons on Purkinje cell dendritic networks also accompanies age-related Purkinje cell involution and loss[15]. Aging also evokes deficits in Purkinje cell function, including marked slowing of firing rates[16] and blunted responses to adrenergic stimulation[17]. These changes have been correlated with impairment in mobility-related behaviors, including walking on a runway and dynamic balance[18].
    Objectivelink
    To further explore potential synaptic deficits underlying age-related gait impairments, we assessed whole-cerebellum neurotransmitter and excitatory amino acid content in cohorts of young and aged C57BL/6 mice using HPLC mass spectroscopy (HPLC-MS). We report a novel finding of increased whole cerebellum serotonin in aged, compared to young, C57BL/6 mice.
    Results & Discussionlink
    Results
    All samples were suitable for HPLC-MS determination of cerebellar neurotransmitter and amino acid levels: glutamate, GABA, glycine, glutamine, dopamine, norepinephrine, tryptophan, serotonin, alanine, threonine, and asparagine. Our results suggest a 1.4-fold statistically significant increase in whole-cerebellar serotonin in aged C57BL/6 mice compared to young conspecifics (Figure a). We otherwise found no other differences in whole cerebellum neurotransmitter or amino acid concentrations between the young and old mouse cohorts.
    Discussion
    Serotonin is an ancient regulator of cellular function[19], and has a coordinating role in the performance of movement behaviors across a wide variety of organisms[20][21][22][23]Aging has a clear impact on cerebellar serotonergic function. The activity of tryptophan hydroxylase, the enzyme catalyzing the rate-limiting conversion of tryptophan to serotonin, is significantly decreased in the midbrain and pons of aged rats[24][25]. Concordantly, age-associated decreases in whole cerebellar serotonin have been reported in rats (assayed by a fluorometric reaction between serotonin and o-phthaldialdehyde[26]) and senescence-accelerated mice (assessed by 3H-tryptophan injection[27]). However, no age-associated differences in cerebellar serotonin content were noted among cohorts of 6-24-month-old BALB mice (assayed by HPLC[28]), and our study is the first to report an age-associated increase in cerebellar serotonin content in a mouse model. Multiple studies have also demonstrated a marked loss of serotonin reuptake transporter function in the cerebellum of older adults[29][30].
    Serotonin has its most profound effect on the Lugaro inhibitory interneurons of the internal granule cell layer. These cells are numerous, have strong inhibitory projections onto Golgi cell inhibitory interneurons, and are typically silent. Lugaro cells respond to serotonin (likely through a 5-HT
    2 subfamily receptor) with a marked increase in spiking activity leading to increased inhibitory postsynaptic potentials onto Golgi cell membranes[31][32]. Lugaro cell activity also directly inhibits Purkinje neuron firing[33]. A single Lugaro cell makes inhibitory synaptic contact with about 150 nearby Golgi cells[31]; each Golgi cell makes inhibitory synaptic contact with about 3,000-5,000 granule cells[34]. Lugaro cell activity thus has the potential to reduce both the tonic and phasic components of Golgi cell inhibition onto large populations of granule cells. Serotonergic neurotransmission also contributes more subtle effects on cerebellar input signal processing. Serotonin attenuates parallel fiber input onto Purkinje cells (both by increasing activity of inhibitory basket and stellate interneurons and by presynaptic decreases of granule cell glutamate[35][36]). Serotonin also modulates cerebellar deep nuclei function in a complex, incompletely understood manner[37].
    Limitationslink
    Our results suggest an age-related 1.4-fold increase in whole cerebellar serotonin content. The methods we employed do not allow us to further determine if this difference reflects increased intravesicular serotonin within cerebellar serotonergic projections, and/or increased extracellular serotonin. Similarly, we cannot determine if this increase occurs over the cerebellum as a whole, or preferentially affects either the cerebellar input regions (internal granule cell, molecular, and Purkinje layers) or the cerebellar output nuclei; nor can we determine whether this finding localizes to specific folia.
    Conjectureslink

    The predicted outcome of increasing cerebellar serotonergic activity may thus be to increase granule cell input sensitivity to mossy fiber input, decrease Purkinje cell sensitivity to parallel fiber input, and increase inhibition of Purkinje cell firing. These effects would combine to expand mossy fiber input participating in center-surround inhibition[38][39]. In other words, increased serotonergic activity may allow a larger set of afferent sensory inputs concurrent access to the Purkinje cell network while preserving overall Purkinje cell firing properties. Of note, in normal human volunteers, increased extracellular serotonin (through a single dose of a selective serotonin uptake inhibitor) evoked a significant increase in resting-state fMRI centrality measures across the cerebellum, consistent with the hypothesis that increased serotonergic activity enhances cerebellar functional connectivity[40].

    It is thus interesting to note in this context that inhibition of serotonergic tone (by treatment with the partial 5-HT1AR agonist buspirone) improved cerebellar tremor[41][42]. Further, prominent cerebellar ataxia has been observed in aged (21-24 monthold) compared to young (2-3 months old) C57BL/6 mice obtained from the same colony as the mice in this study[10]. Interestingly, this appeared to be strain-specific with no ataxia present in BALB mice of identical ages. Locomotor ataxia thus accompanies the increased cerebellar serotonin content observed in C57BL/6 mice, but not in BALB mice with no age-related changes in cerebellar serotonin content[28]. Gait ataxia may potentially be a behavior evoked by serotonergic increases in cerebellar functional connectivity. Further studies to determine if age-associated increases in cerebellar serotonin content are reflected in extracellular serotonin concentrations, and to determine the specific serotonin receptor subtypes associated with Lugaro cell activation, will better define the mechanisms through which serotonergic activity may influence cerebellar tremor, as well as identify potential therapeutic targets.
    Methodslink

    Animals

    All studies were performed in accordance with the NIH Guide for the Care and Use of Laboratory Animals and were approved by our Institutional Animal Care and Use Committee. Six young male C57BL/6J mice were obtained from Jackson Laboratories at 8 weeks of age. Six old (20 months) male C57BL/6 mice were obtained from the National Institute of Aging rodent facility. Animals were housed in our vivarium for 1 month in microisolator cages (Lab Products Inc., Seaford, DE). Mice experienced a 12:12 lighting cycle (lights on 0600 CST) and were provided with chow (Envigo Teklad #7012) and water ad libitum.

    Tissue processing

    Mice were euthanized with acute CO2 asphyxiation, followed by rapid decapitation. Brain was isolated from the skull and placed into ice-cold PBS (Hyclone SH30256.01). Under a dissecting microscope, cerebellum was separated from brain and flash-frozen in isopentane for 5-10 s. Frozen cerebellums were stored at -80°C in canonical tubes until shipping, at which time the tissues/tubes were packed in dry ice and shipped overnight to Brains Online for further analysis.

    Sample preparation

    Mouse cerebella were homogenized using a Misonix Sonicator 3000 ultrasonic cell disruptor (Qsonica, LLC, Newton, USA). Per mg cerebellum 4 μL of 0.5 M perchloric acid was added as tissue destruction matrix. After homogenization, the samples were spun down and supernatant was processed further for the quantification of glutamate, GABA, glycine, glutamine, tryptophan, dopamine, norepinephrine, serotonin, alanine, asparagine, and threonine.

    Analysis

    Supernatant was further processed and 20 μL of the processed sample was mixed with 4 μL stable labeled internal standards mix of the analytes of interest. The mixture was derivatized with the proprietary SymDAQ reagent (BrainLink, Groningen, NL) in the autosampler part of an integrated LC system (prominence series; Shimadzu, Japan) by addition of 30 μL SymDAQ reagent solution to the sample vial. After the reaction, 45 μL of the mixture was injected onto the HPLC-MS system. Chromatographic separation was performed on a reverse-phase column (100×3.0 mm, 2.5 μm particle size; Synergi MAX-RP, Phenomenex, Torrance, USA) at 30°C. Sample analytes were separated using a 100 to 0% gradient of mobile phase A (ultrapure water/acetonitrile (98/2), 0.1% formic acid) to mobile phase B (ultrapure water/acetonitrile (30/70), 0.1% formic acid) at a flow rate of 300 μl/min. A post-column make-up flow of 150 μL/min consisting of 90% acetonitrile and 10% water, was added to the flow of the HPLC, prior to entering the MS for analyte detection. MS analysis was performed using a system consisting of an API 4000 triple quadropole detector and a Turbo V Ion Spray interface (AB Sciex, Redwood City, USA). The acquisitions were performed in positive ionization mode, with ionization spray voltage set at 3 kV and a probe temperature of 200°C. The instrument was operated in multiple reaction monitoring mode. The collision gas (nitrogen) pressure was held at 2 psig. Data was calibrated and quantified using the Analyst™ version 1.4.2 data system (AB Sciex, Redwood City, USA).

    Statistical analysis

    Neurotransmitter/amino acid concentrations were compared over the two cohorts using one-way analysis of variance, with Bonferroni corrections applied to adjust the critical p value for eight simultaneous comparisons (we did not include alanine or threonine in our calculations since these amino acids are not neurotransmitters or neurotransmitter precursors in the cerebellum). Statistics were calculated using MATLAB (anova1; Mathworks, Natick, MA).

    Funding Statementlink
    This study was supported by NIH/NIA R01-AG031158 to SJB.
    Acknowledgementslink
    The authors thank Ms. Tammy Chaudoin for her expert technical support.
    Ethics Statementlink
    Not applicable.

    No fraudulence is committed in performing these experiments or during processing of the data. We understand that in the case of fraudulence, the study can be retracted by Matters.

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