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Observation Type
Continuing the storyline
Oct 18th, 2016
May 22nd, 2017
  • Abstract

    Sensorimotor learning crucially depends on the ability to acquire a sensory memory for shaping motor commands. Such learning is conveniently studied in young songbirds when they memorize the song of an adult singer and gradually transform their own vocalizations toward the memorized target song. Here we study the involvement of the Caudal Medial Nidopallium (NCM), a higher auditory cortical area, in acquisition of a song memory. NCM has previously been shown to be involved in tutor song memorization. To study the necessity of NCM in this process, we perform large irreversible NCM lesions using ibotenic acid injections in about 40 days old juvenile zebra finches, before their first exposure to tutor song. Surprisingly, NCM-lesioned juveniles successfully copied the tutor song at least as well as untreated control animals, showing that a fully intact NCM is not required for tutor song memory formation and normal song development.

  • Figure
  • Introduction

    Similarly to speech learning in humans, developmental birdsong learning involves perception and memorization of a sensory stimulus within a critical sensory period. In zebra finches, this critical period lasts until about 60 days-post-hatch (dph). During this period, a young bird must be exposed to a singing adult bird to develop a normal song. The neuronal substrate for storing this tutor song memory is still largely unknown.

    Many brain areas are involved in tutor song memorization, among which are the premotor nucleus HVC and the caudal medial nidopallium (NCM). HVC is important for the temporal control of birdsong. Optogenetic, electrical, or pharmacological disruption of neural activity in HVC specifically during exposure to tutor song prevents successful tutor song copying. With regards to NCM, there exists both correlative and causal evidence for its involvement in song memorization. The correlative evidence comes from studies of NCM gene expression and electrophysiology. The causal evidence comes from pharmacological manipulations, which reveal that transiently suppressing the extracellular signal-regulated kinase signaling pathway in NCM, specifically during tutoring, severely impairs song learning.

    To probe for possible behavioral differences between transient and irreversible brain manipulations, we test whether NCM neural circuits are required in their full integrity for successful memorization of tutor song. We perform large irreversible and bilateral lesions in NCM of very young birds before we start tutoring them. Thereafter, we examine the juveniles' developing songs and quantify their similarity with tutor song. We compare our findings to normally developing songs in a control group of birds that do not receive any lesions.

  • Objective

    We test whether a fully intact NCM is needed for formation of a song memory during an early phase of song development. In 43 days old juvenile zebra finches we study the effects of very large ibotenic acid injections into NCM. The lesions are made before tutoring onset and before closure of the sensory song learning phase (around 60–80 dph).

  • Results & Discussion

    We made bilateral ibotenic acid lesions in NCM of song-isolate birds when they were 42.5 days old (range 39 to 45, N = 12 birds) and thereafter we exposed the juveniles daily to a singing adult tutor (see Methods). At the end of the experiments (mean 76.3 dph, range 72 to 82 dph, N = 12 birds), juveniles with NCM lesions showed a high song stereotypy (mean 66.9 ± 11.9, range 36.1 to 77.3, N = 12), comparable to the stereotypy in age-matched untreated control juveniles (60.9 ± 7.6, range 53.9 to 70.9, N = 5, p = 0.32, t-test, Panel B).

    NCM-lesioned birds successfully imitated tutor song motifs with an average similarity score of 50.7 ± 10.9 (range 29.1 to 65.3, N = 12 birds), which was not significantly different from similarity in control birds and their tutors (average score 45.1 ± 13.0, range 26.8 to 58.3, N = 5 birds, p = 0.37, t-test). None of the individual measures were significantly different between lesioned birds and control birds: percent similarity (p = 0.61, t-test), sequential match (p = 0.08, t-test), and accuracy (p = 0.81, t-test). NCM-lesioned birds specifically imitated the songs of their tutors and no other songs: The average motif-similarity score in NCM-lesioned birds with their tutors (50.7 ± 10.9 ) was much higher than the average score of 36.4 ± 6.0 with 14 unfamiliar adult birds (range 29.7 to 49.0, N = 12, p = 0.0006, t-test, Panel B).

    We did not find evidence that song learning happened mainly on the days on which the tutor was present with the juvenile. We tested whether pupils’ songs improved in the absence of direct interactions with the tutors. In 4 birds (3 with lesions and 1 control) that were tutored for only 7 days, we calculated tutor-pupil motif similarity scores both on the first day after tutoring offset (53 dph) and at the end of the experiment (75–77 dph). We found that during this period of isolation, the song motifs in all 4 pupils became more similar to their tutors’ motifs than to motifs in 14 unfamiliar adult birds, panel D and E. Thus, birds could improve songs without the presence of their tutors, implying that they used a memory of tutor song that they acquired within a week after the bilateral lesions in NCM.

  • Conclusions

    To our surprise, NCM lesions did not impair tutor song learning, showing that tutor song memory formation does not require a fully intact NCM. These findings contrast with findings presented in where impaired song learning was reported following a more subtle (transient) manipulation in NCM. Given that London et al. injected identical volumes of pharmacological agent as we did, it is unlikely that our manipulation affected smaller portions of NCM than were affected in their study (note that we are not aware of any NCM-manipulation study in which injected volumes were larger than in our study). A possible explanation for the discrepancy with could be an indirect effect of the transient manipulation, triggering a homeostatic imbalance of downstream circuits, as reported infor a different brain area. That is, the impact of transient inactivation of NCM on song learning could be a result of disrupting downstream areas instead of a more direct involvement of NCM in tutor song memorization. For example, tutoring could normally trigger a genetic program causing NCM neurons to change their firing mode, e.g. from spiking to bursting. Downstream neurons would only form a long-term song memory if they receive bursting input from NCM (explaining result from), but when NCM does not support any spiking at all (our study), then reliance on bursting is dropped. According to this view, the effect of transient inactivation may not be directly caused by the inactivation of the targeted area itself, but by the effect on a downstream area (not to provide spike-burst input).

  • Alt. Explanations

    We discuss the possibility that rapid brain repair mechanisms, and in particular neurogenesis, could have restored NCM function before cessation of the tutoring period. Previous studies have shown that new neurons can in principle functionally integrate into existing circuits, as demonstrated in both the songbird HVC and the mammalian hippocampus. Thus, neurogenesis, particularly in the highly plastic juvenile brain, could potentially help to restore damaged NCM tissue and lead to recovery of some of the functions lost through the lesions.

    Our experiments require that hypothetical repair mechanisms must be able to restore NCM circuits within less than a week. The reason for this narrow time window is that in 3 NCM-lesioned birds (Panel D and E) we have observed good song copying even though they were tutored for only seven days after the lesions. The question then boils down to the following: How much brain repair at the level of neural circuits is possible within a week?

    The current literature suggests that neurons integrate into circuits on a slower time scale than this one week limit. First, kainic acid (which like ibotenic acid binds to glutamate receptors) induces rapid, synchronous neuronal death after which there is no neutrophil recruitment and a delay of at least 48 h before macrophage-microglial cell numbers increase. Second, estimates in the hippocampal system show that after more than 7 days, the newly formed granule cells exhibit only a simple unbranched dendrite that does not extend beyond the mitral cell layer. It is generally assumed that it takes more than a month for new granule cells to become fully mature, in line with similar timelines found also in other systems. In combination, we think that there is currently only a small chance that within one week newborn neurons could migrate to NCM and store a detailed auditory memory that they later communicate to motor areas to guide song learning.

  • Methods


    We used a total of 33 male zebra finches (Taeniopygia guttata) from our breeding facility. 12 juveniles were lesioned and 5 were used as controls. Another 16 adult males were used either as tutors or as unfamiliar males (one of the birds was excluded from the analysis because of low motif self-similarity score). Birds were normally raised with both their parents until 15 dph, which is before the onset of the sensory phase in which a memory of the target song is acquired. At 15 dph, the young birds were transferred together with their mothers to a different room without singing males; thus, after 15 dph they were raised without song exposure (note that young zebra finches need to be fed by at least one of the parents until about 30 dph; only afterwards can they be housed individually). Control and NCM-lesioned birds were housed individually in a sound isolation chamber on a 14/10 h day/night schedule. Starting from 1–5 days after surgery, lesioned birds (N = 12) were tutored by placing an adult singing male zebra finch in the same recording chamber 24 h/day. The corresponding controls (N = 5) were identically tutored with tutoring onset at a comparable age (43–46 dph). Birds were tutored on average for 20.6 ± 8.4 days (range 7 to 28 days, N = 17). We did not find a significant correlation between motif similarity scores and the number of days the birds had been exposed to a singing tutor (Pearson's correlation coefficient 0.22, p = 0.40, N = 17 including 5 controls). All but 3 birds were raised in a recording chamber alone with the tutor. 3 birds from one clutch (2 with lesions and 1 control) were instead raised together with a tutor in a large cage. Tutor song similarity in these 3 birds fell within the range of that of the other birds; we therefore included these birds in the analysis.


    Bilateral excitotoxic lesions targeting NCM were made as previously described. Birds were anesthetized using isoflurane and head fixed in a stereotaxic apparatus. In each hemisphere we injected 500 nl ibotenic acid solution (7 mg/ml in ddH2O). Injections were made between 1500 and 2500 μm ventrally from the brain surface at 760 ± 91 μm anterior to the bifurcation of the mid-sagittal sinus and 452 ± 91 μm lateral from the midline (N= 12 birds). Because we found uncompromised learning in lesioned birds, we decided not to perform sham injections in control birds. All birds resumed normal behavior a few hours after surgery. Canopoli et al. previously showed that this lesion protocol effectively lesions a volume in NCM comparable in size to the volume of the injected acid (Panel A).


    To assess lesion extent in histological brain sections we perfused NCM-lesioned birds between 75 and 83 dph (average 78.6 ± 2.5 dph, N = 10 birds). Birds were given an overdose of sodium pentobarbital, followed by a perfusion via the left ventricle first with ringer solution, then with a 4% paraformaldehyde solution. 2 birds were perfused at 143 dph. In these 2 birds and in one control bird, we recorded songs at 120 dph and found a mere 3.9% increase of average similarity score relative to their average score measured at 75 dph (N = 3 birds). Thus, we expect that the songs recorded at 75 dph are good approximations of the songs that juveniles would have produced as adults.

    Song motif selection and analysis

    We selected and analyzed song motifs as described in. Song motifs of juveniles were taken at an age of 76.2 ± 3.0 dph (range 72 to 82). The motif similarity scores between NCM-lesioned birds and their tutors did not correlate with the age of the juveniles on which their songs were recorded (Pearson's correlation coefficient 0.24, p = 0.44, N = 12 birds).

  • Funding statement

    This work was funded by the Swiss National Science Foundation (grant 31003A_127024) and by the European Research Council under the European Community's Seventh Framework Programme (ERC Grant AdG 268911 FP7/2007-2013).

  • Acknowledgements

    We thank Anna Stepien and Homare Yamahachi for helpful comments on the manuscript, and Franziska Baumann-Klausener for technical assistance.

  • Ethics statement

    All the experimental procedures were in accordance with the Veterinary Office of the Canton of Zurich.

  • References
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    Bilateral neurotoxic lesions in NCM before tutoring onset do not prevent successful tutor song learning

    Affiliation listing not available.
    Continuing the storyline of

  • Canopoli Alessandro, Zai Anja, Hahnloser Richard
    Lesions of a higher auditory brain area during a sensorimotor period do not impair birdsong learning
  • Peer-reviewed by 2 reviewers with median rating of 12.5/20. Review process was triple-blinded.

    Round 1 (12/20)
    Round 2 (12.5/20)
    Technical quality7
    Conceptual advance and Impact8

    General comment

    Results & Discussion
    Technical quality4
    Conceptual advance and Impact4.5

    General comment

    Results & Discussion