In this work, the authors applied antibody tau2 which is directed against both phosphorylated and unphosphorylated tau species. No double immunofluorescence stainings assessing the distribution of tau and BIN1 in one single section are presented beside "co-stainings" using unspecific Thio S beta-sheet staining. The authors should address this issue to support their results given the possibility that tau may still colocalize with BIN1, e.g. in a pretangle state (which may histologically very much display a "punctate" pattern). Ideally, additional tau antibodies specifically directed p-tau may be applied (although in the reviewer's opinion not a prerequisite for publication).