Your browser is out-of-date!

Update your browser to view this website correctly. Update my browser now

×

Discipline
Biological
Keywords
Synaptic Integration
Synapses
Neurogenesis
Hippocampus
Synaptic Transmission
Observation Type
Standalone
Nature
Standard Data
Submitted
Sep 22nd, 2016
Published
Dec 29th, 2016
  • Abstract

    Dentate granule cells are born throughout life in the mammalian hippocampus. The integration of newborn neurons into the dentate circuit is activity-dependent, and structural data characterizing synapse formation suggested that the survival of adult-born granule cells is regulated by competition for synaptic partners. Here we tested this hypothesis by using a mouse model with genetically enhanced plasticity of mature granule cells through temporally controlled expression of a nuclear inhibitor of protein phosphatase 1 (NIPP1*). Using thymidine analogues and retrovirus-mediated cell labeling, we show that synaptic integration and subsequent survival of newborn neurons is decreased in NIPP1*-expressing mice, suggesting that newborn neurons compete with pre-existing granule cells for stable integration. The data presented here provides experimental evidence for a long-standing hypothesis and suggest cellular competition as a key mechanism regulating the integration and survival of newborn granule cells in the adult mammalian hippocampus.

  • Figure
  • Introduction

    Neural stem/progenitor cells (NSPCs) generate the vast majority of neurons in the brain during embryonic development. However, the neurogenic capacity of NSPCs does not end with birth as new neurons are born across the entire life-span in discrete areas of the mammalian brain. One of these regions is the hippocampal dentate gyrus (DG) where NSPCs persist throughout life and continuously generate new granule cells, the principal neuronal subtype of the DG. Newborn neurons are critically involved in a number of hippocampus-dependent cognitive functions including behavioral pattern separation, forgetting, and mood regulation. Moreover, failing or altered neurogenesis has been associated with several diseases such as major depression and epilepsy, suggesting that the addition of new neurons into the DG circuitry has translational relevance. Similar to embryonic development, a surplus of neurons is initially generated in the adult brain. The survival and stable integration of new neurons appears to be regulated in a two-step process: whereas a substantial number of newborn neurons dies within the first days after their birth, there is a second phase of selection that occurs approximately 1-3 weeks after neuronal birth. Selection during this stage depends on N-methyl-D-aspartate (NMDA) receptor-mediated activity as conditional deletion of the GluN1 (NR1) subunit in newborn granule cells substantially decreases the number of surviving neurons. During the phase of integration, newborn granule cells are highly excitable and remain so for approximately 6 weeks. This period of high cellular plasticity has been associated with unique functional properties of new neurons and may help new neurons to successfully integrate into the pre-existing circuit. Indeed, it has been suggested that new neurons compete for synaptic partners allowing for stable integration and subsequent survival. We here tested this hypothesis by examining whether the cell type-selective enhanced plasticity of mature granule cells affects integration and survival of newborn granule cells. Strikingly, we have found that enhancing the plasticity of the mature dentate granule cell circuit leads to decreased survival of newborn granule cells, providing experimental evidence that competition may be a critical component for the survival of newborn granule cells.

  • Objective

    We investigate the survival and morphology of newborn granule cells in the adult dentate gyrus in a model of enhanced plasticity of mature neurons to test the hypothesis that a mechanism of competition governs integration into the neuronal circuits.

  • Results & Discussion

    Expression of a nuclear inhibitor of PP1 (NIPP1*) in mature granule cells

    To test for evidence of activity dependent competition for survival between new neurons and pre-existing granule cells, we used a mouse model where the plasticity of mature granule cells is enhanced through transgenic expression of a constitutively active form of the nuclear inhibitor of protein phosphatase 1 (NIPP1*). NIPP1* expression in mature granule cells has been previously shown to enhance synaptic plasticity of granule cells in the adult DG, leading, for example, to an increased amplitude of long-term potentiation (LTP). To selectively direct NIPP1* expression to mature granule cells, we used a cell type specific approach where NIPP1* is expressed under the control of the CaMKIIα promoter and the reverse tetracycline (Tet)-controlled transactivator 2 (CaMKIIα-driven rtTA2 x TetO-NIPP1*/EGFP; hereafter called tg-NIPP1*; Fig. 1A). We found that the CaMKIIα promoter is not active in newborn granule cells expressing the microtubule-associated protein doublecortin (DCX) that is transiently expressed in newborn neurons but not in mature granule cells, and that transgene expression upon Doxycycline (DOX) treatment was highly selective in mature granule cells (NeuN+, DCX-) in the DG as measured by expression of EGFP fused to NIPP1* (Fig. 1A, and data not shown). Thus, transgenic NIPP1* expression within the DG is restricted to mature granule cells past the expression stage of DCX making this model suitable for testing the effects of enhanced mature granule cell plasticity on NSPC proliferation, fate determination, and stable integration of newborn neurons.

    Tg-NIPP1* expression in mature granule cells does not affect NSPC proliferation

    As previous reports suggested that NSPC proliferation is regulated by neuronal activity, we first analyzed if NSPC proliferation is affected upon tg-NIPP1* expression. We compared DOX-fed tg-NIPP1* mice with their single transgenic (lacking the TetO-NIPP1*/EGFP transgene) and non-DOX-fed tg-NIPP1* littermates as controls. Non-DOX-fed single as well as double transgenic mice and DOX-fed single and double transgenic mice were analyzed separately to exclude leakiness of the transgene expression and to test for a potential influence of DOX alone. We did not observe significant differences in the rate of proliferation and number of newborn neurons as measured using DCX expression and thymidine analogue labeling (BrdU, CldU, and IdU) between any of the control groups in any experiment (Suppl. Fig. 1A, B). Upon 2 weeks of DOX treatment, NSPC proliferation was analyzed using a single BrdU pulse (Fig. 1B) 14 h prior to killing the animals. Using this approach, we observed no significant difference between DOX-fed tg-NIPP1* and their respective controls (Con: 1332 ± 145; DOX-tg-NIPP1*: 1340 ± 125 BrdU+ cells per DG, n.s). We next analyzed if tg-NIPP1* expression affects early neuronal cell death or fate specification of newborn cells. Using the thymidine analogue IdU, we labeled cells 1 week prior to analyses and found no difference between tg-NIPP1* mice and respective controls (Fig. 1C) (Con: 1184 ± 163; DOX-tg-NIPP1*: 1138 ± 122 IdU+ cells per DG, n.s.). In addition, we found that virtually all IdU-labeled cells expressed the neuronal markers DCX and Prox1 (Fig. 1C, and data not shown), suggesting that fate determination and differentiation of newborn neurons is not affected in DOX-tg-NIPP1* mice.

    Enhanced plasticity of the mature granule cell circuit impairs survival of newborn neurons

    We then tested if the total number of immature neurons in tg-NIPP1* mice is affected upon DOX treatment. DCX starts to be expressed in late dividing NSPCs and expression lasts for approximately 3 weeks after neuronal birth. Strikingly, the number of DCX-expressing cells was substantially reduced in DOX-tg-NIPP1* mice (Con: 13182 ± 662.2; DOX-tg-NIPP1* 9810 ± 828.8 DCX+ cells per DG, p <0.05), indicating a loss of immature neurons at later stages when activity dependent survival occurs (Fig. 1D). We used a complementary approach to confirm the loss of new neurons based on DCX analyses and injected the thymidine analogue CldU in DOX-tg-NIPP1* mice and respective controls. Animals were killed 3 weeks later and the number of CldU+ cells analyzed. Corroborating the DCX results, we found a significant drop in CldU-labeled cells in DOX-tg-NIPP1* mice compared to controls (Con: 808 ± 100 control; DOX-tg-NIPP1*: 465 ± 101 CldU+ cells per DG, p <0.05) (Fig. 1E). Interestingly, these results suggest that neuronal loss occurs around the time when newborn neurons start to form dendritic spines and excitatory synapses suggesting that synaptic competition, impaired by enhanced plasticity of the mature granule circuit through NIPP1* expression, may be critical for stable integration into the DG network.

    Reduced dendritic complexity in newborn neurons in tg-NIPP1* mice

    We next analyzed if the length and branching of newborn neurons are affected in DOX-tg-NIPP1* mice, using these measures as a proxy for neuronal integration. To analyze the morphology of newborn neurons, we injected retroviruses expressing GFP under chicken beta-actin promoter stereotactically into the DG of control and tg-NIPP1* mice and killed the animals 3 weeks later. Using this approach, we found that dendritic length was significantly reduced in DOX-tg-NIPP1* mice compared to controls (Fig. 1F) (Con: 476.8 µm ± 18.5; DOX-tg-NIPP1*: 368.1 µm ± 22.8 average dendritic length, p <0.001). Further, dendrites extending from neurons born in DOX-tg-NIPP1* mice had substantially fewer branches compared to controls (Fig. 1F) (Con: 8.43 ± 0.52; DOX-tg-NIPP1*: 6.14 ± 0.35, branch points per neuron, p <0.01). In contrast, we found that axonal growth into area CA3, which is reached by axons extending from newborn neurons before first spines are formed was not altered in DOX-tg-NIPP1* mice (Suppl. Fig. 2) (Con: 1122.74 ± 91.73 µm; DOX-tg-NIPP1* 1225.73 ± 42.36 µm, n.s.). After finding that dendrites extending from newborn neurons in DOX-tg-NIPP1* were shorter and less complex, we next analyzed the number of dendritic spines, the main place for excitatory synapses of excitatory neurons, in DOX-tg-NIPP1* and control mice (Fig. 1G). On the dendritic segments analyzed, the number of spines per µm dendrite was similar between groups (Con: 0.469 ± 0.04; DOX-tg-NIPP1*: 0.423 ± 0.02 spines/µm, n.s.) (Fig. 1G). Furthermore, the subtype of spines did not differ between DOX-tg-NIPP1* and control mice (Con: 0.761 ± 0.07; DOX-tg-NIPP1*: 0.898 ± 0.07 mushroom spines/µm; Con: 0.136 ± 0.01; DOX-tg-NIPP1*: 0.105 ± 0.09 stubby spines/µm; Con: 0.256 ± 0.03; DOX-tg-NIPP1*: 0.227 ± 0.02 thin spines/µm, n.s.). However, given that dendrites are shorter in DOX-tg-NIPP1* mice, we reasoned that despite similar spine density the total number of excitatory inputs is reduced per newborn neuron in DOX-tg-NIPP1* mice compared to controls. To estimate the number of spines per cell, we multiplied spines per µm with the calculated dendritic length. It has to be considered, however, that spines are not uniformly distributed on the dendrites; so any calculations can only be a rough estimation (e.g., within the granule cell layer (GCL), only few spines are formed). Given that dendrites are substantially longer in controls than in DOX-tg-NIPP1* mice, we estimated a reduction of synaptic input of approximately 50% of newborn neurons in DOX-tg-NIPP1* compared to controls (195.7 in control, 130.6 in DOX-tg-NIPP1* calculated spines per cell). Next, we analyzed synapse formation of new neurons in DOX-tg-NIPP1* on the ultrastructural level. Again, we used retroviruses expressing GFP to label newborn neurons in DOX-tg-NIPP1* mice and controls. Synapse formation was analyzed 3 weeks after stereotactic injection of retroviruses and after immunhistochemical conversion of the GFP signal into osmiophilic DAB precipitate. We then used focused ion beam scanning (FIBS)- electron microscopy (EM) to reconstruct spines and their environment in 3-dimensions (n = 1 mouse per genotype). In total, we analyzed 73.2 µm of 2 dendrites containing 36 synapses in DOX-tg-NIPP1* (n = 1). Using this approach, we found that spines of newborn neurons in DOX-tg-NIPP1* mice formed synapses with visible postsynaptic densities, indicating functional connectivity, and engaged in multiple synapse boutons (MSBs), suggesting that synapse formation is not fundamentally altered in DOX-tg-NIPP1* mice (Fig. 1H).

    We used a genetic approach to test if the survival of new neurons in the adult DG is influenced by the plasticity of the mature granule cell circuit. Strikingly, we found that dendritic integration and subsequent survival of newborn granule is impaired with enhanced plasticity of the mature granule cell circuit, suggesting that competition for synaptic integration is critical in regulating the survival of new neurons. New neurons need to receive synaptic input for their survival, and structural data suggested that filopodia extending from dendrites of newborn neurons initially grow towards existing synapses between mature granule cells and presynaptic axons in the perforant path originating from neurons in the entorhinal cortex, leading to the formation of MSBs. With time, MSBs are then presumably exchanged by single synapse boutons (SSBs) suggesting that new neurons that successfully compete for synaptic partners stably integrate. The survival of newborn neurons can be enhanced, for example, by environmental enrichment that appears to enhance excitability in the DG circuit. Similarly, the reduced survival of new neurons lacking the GluN1 subunit of the NMDA receptor can be partially rescued by global blockade of NMDA-dependent activity. However, characterizing a potential competition by manipulating the balance of excitability selectively between new and mature granule cells had not been tested experimentally. We achieved this by cell type-specific expression of NIPP1* in mature granule cells and found that enhancing plasticity of the mature granule cell circuit impairs the survival of newborn granule cells. The growth of dendrites extending from newborn granule cells was substantially impaired in NIPP1*-expressing mice, whereas synapse formation appeared to be unaltered as analyzed using conventional light microscopy and electron microscopy. This led to a strongly reduced number of dendritic spines of newborn neurons, suggesting that their overall excitatory synaptic input is decreased. Since the transgene is expressed in all CaMKII-expressing cells, including those of the entorhinal cortex, we cannot exclude an effect of more globally changed activity of neuronal circuits. Without longitudinal imaging of synapse formation, which is currently technically not feasible at the required resolution, it cannot be proven that new neurons fail to survive due to impaired synaptic competition. However, our data strongly supports the hypothesis that new neurons need to compete for synaptic partners to ensure proper integration and survival. Interestingly, it has been shown that during the first 3–6 weeks after their birth, new granule cells are highly excitable and show a higher degree of plasticity compared to mature granule cells. This unique feature has been attributed to their special functional properties with emerging evidence supporting the idea that adult neurogenesis in the DG is not a process for mere cell replacement but that new neurons exert their function at least partially due to these special properties. However, it is also reasonable to speculate that the phase of heightened excitability may also be important to ensure the integration of new neurons, giving them a competitive advantage to form synapses with axons arising from the entorhinal cortex. Our data supports this idea by showing that the survival of new neurons is impaired with increased plasticity of the mature granule cell circuit, whereas NSPC proliferation and initial steps of fate determination and specification are unaltered. Thus, we here experimentally support a long-standing hypothesis, indicating that synaptic competition represents a key mechanism regulating the integration and survival of newborn granule cells in the adult mammalian hippocampus.

  • Limitations

    We used a single mouse model of enhanced plasticity with wildtype littermate controls. While, we interpret our data as a strong suggestion of a competitive mechanism at work, additional studies using different models and approaches are needed to prove that the effects observed are not specific to the model. In our model, the transgene is expressed under the control of the CaMKIIα promoter, which is active in all forebrain neurons. It is possible that, next to the effect of the immediate environment, other changes inherent to the transgene also affect neuronal integration and morphology. One immediate effect on newborn granule cells may be altered input by medial entorhinal cortex cells, the primary input of the hippocampus.

  • Methods

    Animals, doxycycline administration, and stereotactic injection

    All animal experiments were done according to the guidelines of and approved by the veterinary office of the Canton of Zürich, Switzerland. NIPP1*-GFP rtTA2 have been described before. Doxycycline (DOX) (Grovet, Amsterdam) was administered as wet food mixture. 600 mg doxycycline was mixed with 500 g wet food (equals to 100 g dry food) and 20 g wet food with DOX was given per mouse, freshly prepared every day. Mice were stereotactically injected with 1 µl of retroviral suspension, as described before. The coordinates were 2 mm posterior of the bregma, 1.5 mm lateral of the midline, and 2.3 mm ventral from the skull, with bregma and lamda leveled and measures taken at the bregma. Injections were performed using a stereotactic frame (Kopf) and a Hamilton syringe (Hamilton). Animals were anesthetized using Ketamin (Ratiopharm) and Xylazin (Bayer). For BrdU experiments, we used DOX-tg-NIPP1* (n = 3) and non-DOX-tg-NIPP1* (n = 5); for IdU/CldU experiments we used DOX-tg-NIPP1* (n = 7), non-DOX-tg-NIPP1* (n = 4), single transgenic DOX-TetO-NIPP1* (n = 9), single transgenic non-DOX-TetO-NIPP1* (n = 12); for retroviral experiments: DOX-tg-NIPP1* (n = 4) and non-DOX-tg-NIPP1* (n = 4); for EM experiments DOX-tg-NIPP1* (n = 1) and non-DOX-tg-NIPP1* (n = 1). Thymidine analogues BrdU, CldU or IdU (Sigma) were administered as intra peritoneal (i.p.) injections in doses equimolar to 100 mg/kg of BrdU at the noted timepoints. For antigen retrieval, sections were incubated in 2 N HCl at 37°C for 30 min, followed by a wash in 0.1 M Borate buffer. CldU and IdU were detected by antibodies directed against BrdU, one of which (rat α-BrdU, Abcam) recognizes, next to BrdU, also CldU but not IdU; the second one (mouse α-BrdU, BD) recognizes all three thymidine analogues, but the affinity to CldU is weaker than to the other two and it can be washed off with Tris-HCl (40 mM, pH 8.0, 0.5 M NaCl, 1% Tween). That way, if the mouse α-BrdU is first applied to the sections and washed off from the CldU afterwards, followed by a staining with the rat α-BrdU, each antibody specifically detects one of the thymidine analogues.

    Immunohistochemistry

    Mice were perfused transcardially with 0.9% NaCl, followed by freshly prepared chilled 4% formaldehyde solution (pH 7.4). The brains were postfixed overnight on 4% PFA in 4°C on a shaker and transferred to 30% sucrose solution on the next day for cryoprotection. For sectioning, the brains were cut to 40 µm thick coronal sections on a freezing sliding microtome (Microm). Sections were collected to a cryoprotectant solution (CPS, 25% Ethyleneglycol, 25% Glycerin, 0.1 M PO4) in a series of 12. For quantitative analysis, 2 series of each brain were stained and analyzed. For immunohistochemical staining, sections were washed and blocked in 3% donkey serum (Millipore) and 0.2% Triton X100 (Sigma). Primary antibodies used were goat α-DCX (Santa Cruz), rat anti-BrdU (Abcam), mouse α-BrdU (BD), mouse α-NeuN (Millipore), and goat α-Prox1 (Santa Cruz). Secondary antibodies were donkey α-primary antibody species, conjugated to different fluorophores (Jackson ImmunoResearch). For determining the number of thymidine analog positive cells, the cells were counted on all sections of 2 series and the number multiplied by 6. This number was then denoted as the final number of cells per DG.

    Plasmids and viruses

    Retroviral vectors based on a murine Moloney leukemia virus expressing green fluorescent protein (GFP) under the control of the chicken β-actin (CAG) promoter were produced (CAG-GFP) and injected as described previously. Microscopic counting of thymidine analog positive cells was done with a 40× objective on an Zeiss Axiovert Observer-D1 inverted microscope. For measuring the dendritic length, z-stacks were acquired using a 20× objective on the Leica SP2 AOBS laser scanning confocal microscope. A maximum projection of the stacks was measured using ImageJ (NIH) with the NeuronJ plugin for analysis of dendritic length and branch points. For measuring spine density, z-stacks were acquired using the Leica SP2 AOBS with the 63× oil objective and a zoom of 5, a resolution of 1024×1024 pixels, and a z-step size of 0.2 µm. Those stacks were then deconvoluted using Huygens essential deconvolution software (SVI). Dendrites and spines were tracked in semi-3D using NeuronStudio, and the spines were categorized according to their morphology. The data from NeuronStudio was evaluated using a VBS MS Excel Tool for extraction and automated analysis on the collected data for spine numbers, densities, and classifications. Images were processed using Photoshop CS5 (Adobe), ImageJ (NIH), FIJI or Imaris (Bitplane). To estimate the number of spines per cell, the density was multiplied with the dendritic length. It has to be considered, however, that spines are not uniformly distributed on the dendrites. As virtually no spines are formed in the granule cell layer (GCL), we subtracted 60 µm from the average length to exclude the portion of the GCL of the dendrites. The dendritic portion of the GCL is not included in the spine density numbers, as we did not acquire images there. Therefore, we multiplied the measured number of spines/µm with the average measured dendritic length reduced by 60 µm to obtain the estimated number of spines per cell.

    Electron microscopy

    For electron microscopy of synaptic spines, mice were perfused transcardially with 0.9% NaCl and 4% cold PFA, 0.1 M phosphate buffer and post fixed overnight with the brain still in the skull on 4°C, followed by 48 h postfixation of the brain in 4% PFA. The brains were sectioned using a Leica vibratome to 50 µm thick sections. Cells containing GFP were visualized using a rabbit anti-GFP (Chemicon) primary and a biotinylated anti-rabbit secondary antibody (Jackson), followed by a DAB staining (Vectorlabs Kit). Afterwards, lipidous structures were visualized by incubation in 1% OsO4. The sections were then embedded in epon resin for electron microscopy. The tissue section was glued on a resin block. Using a wide-field light microscope, stained dendrites in the embedded section were identified and landmarks created in relation to them on the surface of the section using a syringe needle. The resin block was shortened to about 4–5 mm and mounted with superconductive carbon cement (Leit-C, Neubauer Chemikalien) onto SEM-stubs. The samples were sputter-coated with 5 nm platinum (BalTEC sputter coater: MED 010), and the surface imaged in a scanning electron microscope (SEM) (Zeiss LEO 1230, SE mode). SEM surface images and the light microscopic images were combined and aligned in Adobe Photoshop CS5. The area of interest was relocated in FIB/SEM and covered with up to 1 µm carbon deposition (GIS, FIB/SEM FEI Helios600i). The surface was opened and a perpendicular section plane of about 50×50 µm along the stained neuron was generated by focused ion beam milling (gallium ions at 30 kV, 21 nA, and 9 nA) (Helios Nanolab 600i and Zeiss NVision 40). The surface was polished and sequentially cut with a focused ion beam at 30 kV and 2.5 nA. Between the serial sections, SEM images were taken at 2 kV and 0.17 nA at selected regions within the section plane (back-scattered electron signal of the in-lens detector at a dwell time of 30 µs per pixel using the G3-slice & view software on FEI Helios600i). Each image area was captured with an image pixel size of 5 nm. Images were taken every 30 nm, resulting in a data voxel size of 5×5×30 nm. Serial images were aligned and processed in FIJI. Structures were registered manually using either FIJI TRAK EM or Imaris (Bitplane). From the tracked contours, three-dimensional reconstructions were done using the same programs. Synapses were identified and categorized as single or multiple synapse boutons.

    Statistical analysis

    Statistical analysis was performed using SPSS 18 (IBM). Differences were considered significant at p <0.05. All the tests used to compare the average number of cells, spines, or dendritic length in the control vs. the Dox-fed double transgenic mice were two-tailed independent sample t-tests. Significance indicates the p-value was below 0.05; if above it is reported as non-significant (n.s.),± represents the standard error of the mean.

  • Funding statement

    This study was supported by the EMBO Young Investigator program and the Swiss National Science Foundation (BSCGI0_157859; 31003A_156943) (to SJ).

  • Acknowledgements

    We thank Anne Greet Bittermann for outstanding help with FIBS-EM (ScopeM, ETH Zurich), D. Chichung Lie for critical comments on the manuscript, Thomas R. Simon for programming the tool used for spine analyses, and the ZMB (UZH) and ScopeM for help with imaging.

  • Ethics statement

    All animal experiments were done according to the guidelines of and approved by the veterinary office of the Canton of Zürich, Switzerland.

  • References
  • 1
    Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum

    Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum ipsum Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum ipsum

    Lorem ipsum Lorem ipsum Lorem ipsum
    2
    Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum

    Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum ipsum Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum ipsum

    Lorem ipsum Lorem ipsum Lorem ipsum
    3
    Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum

    Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum ipsum Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum ipsum

    Lorem ipsum Lorem ipsum Lorem ipsum
    4
    Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum

    Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum ipsum Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum ipsum

    Lorem ipsum Lorem ipsum Lorem ipsum
    5
    Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum

    Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum ipsum Lorem ipsum Lorem ipsum Lorem ipsum Lorem ipsum ipsum

    Lorem ipsum Lorem ipsum Lorem ipsum
    Matters Select15.5/20

    Enhanced plasticity of mature granule cells reduces survival of newborn neurons in the adult mouse hippocampus

    Affiliation listing not available.
    Submitted
    Sep 22nd, 2016
    Accepted
    Dec 21st, 2016
    Published
    Dec 29th, 2016
    Time to publish
    3 months
    Discipline
    Biological
    Subdiscipline
    Neuroscience
    Keywords
    Synaptic Integration
    Synapses
    Neurogenesis
    Hippocampus
    Synaptic Transmission
    Observation Type
    Standalone
    Nature
    Standard Data
    DOI
    10.19185/matters.201610000014
    Copy Cite
    PDF
    Available
    Show Download
    XML
    Peer review
    External discussion
    Suppl. Information
    Raw Data
    -
    Figure downloads