In (A), please indicate what the open and closed circles/boxes indicate. (C), needs a legend to show what the different colored vertical bars represent ? same issue in (E) as well. In (D), the nuclear levels of the sensor increases in the control. What does this indicate ? How representative are the images ? Also, a negative control showing that the cytoplasmic export is specific to the JNK and not just a leakage of nuclear proteins due to loss of nuclear integrity. A control GFP or some other non-tagged fluorescent protein will be helpful. This is important because this is the only assay that shows the JNK activation. Also, this observation could be supported by using antibody staining of fixed cells using anti-phospho JNK specific antibody. Also the evidence for whether JNK directly phosphorylates Stathmin is lacking. An in vitro assay could be helpful here.